Boundary Element-Associated Factor 32B Connects Chromatin Domains to the Nuclear Matrix

The Drosophila Boundary Element-Associated Factors BEAF-32A and 32B Affect Chromatin Structure

The Drosophila boundary element-associated factors BEAF-32A and BEAF-32B affect chromatin structure.

Binding sites for the Drosophila boundary element-associated factors BEAF-32A and -32B are required for the insulator activity of the scs' insulator. BEAF binds to hundreds of sites on polytene chromosomes, indicating that BEAF-utilizing insulators are an important class in Drosophila. To gain insight into the role of BEAF in flies, we designed a transgene encoding a dominant-negative form of B...

Visualization of chromosomal domains with boundary element-associated factor BEAF-32

We have purified two proteins from Drosophila that bind to the scs' boundary element of the hsp70 domain at locus 87A7. Their palindromic binding sites (CGATA-TATCG) symmetrically abut the previously mapped hypersensitive site of scs'. We have cloned a cDNA for one of these proteins, BEAF-32 (boundary element-associated factor of 32 kDa). It encodes a novel protein that is bound to scs' but not...

The nuclear pore as a chromatin boundary

he Cajal body (CB, formerly coiled body) is a prominent nuclear structure, yet its function remains obscure. Now, speculate that the CB is where small nuclear RNAs go for modification. Last year, Kiss's team identified a small guide RNA, U85, that directs both 2'-O-methylation and pseudouridylation of the spliceosomal component U5 snRNA. This year, they've identified six more RNAs, Novel guide ...

The scs9 Boundary Element: Characterization of Boundary Element-Associated Factors

Boundary elements are thought to define the peripheries of chromatin domains and to restrict enhancerpromoter interactions to their target genes within their domains. We previously characterized a cDNA encoding the BEAF-32A protein (32A), which binds with high affinity to the scs* boundary element from the Drosophila melanogaster 87A7 hsp70 locus. Here, we report a second protein, BEAF-32B, tha...